Anti-UBE2A Polyclonal Antibody (HB413014)

Name: Anti-UBE2A Polyclonal Antibody
Brand: abinScience
SKU: HB413014
Price: 96 USD
Availability: InStock

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Каталожный номер

HB413014

Описание

Anti-UBE2A Polyclonal Antibody (HB413014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A in ELISA, IHC, WB. Suitable for Human, Mouse, Rabbit, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant human UBE2A (Met1-Cys152).

Цель

Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

P49459, Q9Z255, P63146, P63148, P63149, P63147, Q32P99, P25153, P52478

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Ubiquitin-conjugating enzyme E2 A (UBE2A) is a ~17 kDa protein. E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN.

1. Kim, J. et al. (2005) Molecular cell 20, 759-70. PMID: 16337599

2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

3. Haddad, DM. et al. (2013) Molecular cell 50, 831-43. PMID: 23685073

4. Hong, JH. et al. (2015) Molecular & cellular proteomics : MCP 14, 674-85. PMID: 25582440

5. Haakonsen, DL. et al. (2024) Nature 626, 874-880. PMID: 38297121

6. Barnsby-Greer, L. et al. (2024) Nature structural & molecular biology 31, 351-363. PMID: 38182926

Примечание

For research use only

Изображения

Western blot

Western blot analysis was performed using anti-UBE2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: A375 cell lysate
Lane 5: SW620 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: N2A cell lysate
Lane 8: NIH3T3 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Rat brain lysate

Ссылки

Даташит

Anti-UBE2A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-UBE2A Polyclonal Antibody (HB413014)

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