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Anti-UBE2H Polyclonal Antibody (HB330014)

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Vista general
Número de catálogoHB330014
Descripción
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human UBE2H (Met1-Leu183).
Objetivo Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P62256, P62257, Q32LN1
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Ubiquitin-conjugating enzyme E2 H (UBE2H) is a ~20 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.

1. Hoeller, D. et al. (2007) Molecular cell 26, 891-8. PMID: 17588522
2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
3. Kaiser, P. et al. (1994) The Journal of biological chemistry 269, 8797-802. PMID: 8132613
4. Lampert, F. et al. (2018) eLife 7. PMID: 29911972
Nota For research use only
Imágenes
Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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