Anti-UBE2H Polyclonal Antibody (HB330014)

Name: Anti-UBE2H Polyclonal Antibody
Brand: abinScience
SKU: HB330014
Price: 168 USD
Availability: InStock

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Spécification :

50ug
100ug

Nombre :

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SDS

Aperçu

Numéro de catalogue

HB330014

Description

Anti-UBE2H Polyclonal Antibody (HB330014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human UBE2H (Met1-Leu183).

Cible

Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P62256, P62257, Q32LN1

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Ubiquitin-conjugating enzyme E2 H (UBE2H) is a ~20 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.

1. Hoeller, D. et al. (2007) Molecular cell 26, 891-8. PMID: 17588522

2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

3. Kaiser, P. et al. (1994) The Journal of biological chemistry 269, 8797-802. PMID: 8132613

4. Lampert, F. et al. (2018) eLife 7. PMID: 29911972

Note

For research use only

Images

Reférences

Fiche technique

Anti-UBE2H Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-UBE2H Polyclonal Antibody (HB330014)

Aperçu

Images

Reférences

Fiche technique

SDS