Anti-UBE2I/UBC9 Polyclonal Antibody (HX847014)

Name: Anti-UBE2I/UBC9 Polyclonal Antibody
Brand: abinScience
SKU: HX847014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HX847014

Description

Anti-UBE2I/UBC9 Polyclonal Antibody (HX847014) is a rabbit polyclonal antibody detecting UBE2I in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Danio rerio, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human UBE2I/UBC9 (Asp33-Ser158).

Cible

UBE2I, UBC9, p18, UBCE9, Ubiquitin carrier protein 9, RING-type E3 SUMO transferase UBC9, SUMO-conjugating enzyme UBC9, Ubiquitin carrier protein I, Ubiquitin-protein ligase I, Ubiquitin-conjugating enzyme E2 I, SUMO-protein ligase

Purification

Purified by antigen affinity column.

Numéro d'accès

P63279

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

SUMO-conjugating enzyme UBC9 (UBE2I) is a ~18 kDa protein. Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.

1. Sin, Y. et al. (2016) The Journal of biological chemistry 291, 1387-97. PMID: 26620705

2. Woeller, CF. et al. (2007) The Journal of biological chemistry 282, 17623-31. PMID: 17446168

3. Anderson, DD. et al. (2007) Clinical chemistry and laboratory medicine 45, 1760-3. PMID: 18067453

Note

For research use only.

Images

Reférences

Fiche technique

Anti-UBE2I/UBC9 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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