Anti-UBE2I/UBC9 Polyclonal Antibody (HX847014)

Name: Anti-UBE2I/UBC9 Polyclonal Antibody
Brand: abinScience
SKU: HX847014
Price: 168 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HX847014

説明

Anti-UBE2I/UBC9 Polyclonal Antibody (HX847014) is a rabbit polyclonal antibody detecting UBE2I in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Danio rerio, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human UBE2I/UBC9 (Asp33-Ser158).

ターゲット

UBE2I, UBC9, p18, UBCE9, Ubiquitin carrier protein 9, RING-type E3 SUMO transferase UBC9, SUMO-conjugating enzyme UBC9, Ubiquitin carrier protein I, Ubiquitin-protein ligase I, Ubiquitin-conjugating enzyme E2 I, SUMO-protein ligase

精製

Purified by antigen affinity column.

アクセッション番号

P63279

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

SUMO-conjugating enzyme UBC9 (UBE2I) is a ~18 kDa protein. Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.

1. Sin, Y. et al. (2016) The Journal of biological chemistry 291, 1387-97. PMID: 26620705

2. Woeller, CF. et al. (2007) The Journal of biological chemistry 282, 17623-31. PMID: 17446168

3. Anderson, DD. et al. (2007) Clinical chemistry and laboratory medicine 45, 1760-3. PMID: 18067453

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-UBE2I/UBC9 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-UBE2I/UBC9 Polyclonal Antibody (HX847014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ