Anti-VCP Polyclonal Antibody (HX922014)

Name: Anti-VCP Polyclonal Antibody
Brand: abinScience
SKU: HX922014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
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Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HX922014

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human VCP (Gly125-Ile371).

Objetivo

Transitional endoplasmic reticulum ATPase, Valosin-containing protein, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, VCP

Purificación

Purified by antigen affinity column.

Número de acceso

P55072

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Transitional endoplasmic reticulum ATPase (VCP) is a ~89 kDa protein. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome.

1. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139

2. Kadowaki, H. et al. (2015) Cell reports 13, 944-56. PMID: 26565908

3. Turakhiya, A. et al. (2018) Molecular cell 70, 906-919.e7. PMID: 29804830

4. Gwon, Y. et al. (2021) Science (New York, N.Y.) 372, eabf6548. PMID: 34739333

5. Meerang, M. et al. (2011) Nature cell biology 13, 1376-82. PMID: 22020440

6. Acs, K. et al. (2011) Nature structural & molecular biology 18, 1345-50. PMID: 22120668

7. Mosbech, A. et al. (2012) Nature structural & molecular biology 19, 1084-92. PMID: 23042605

8. Davis, EJ. et al. (2012) Nature structural & molecular biology 19, 1093-100. PMID: 23042607

9. Fielden, J. et al. (2020) Nature communications 11, 1274. PMID: 32152270

10. Krastev, DB. et al. (2022) Nature cell biology 24, 62-73. PMID: 35013556

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-VCP polyclonal antibody at 1ug/mL on various samples.
Lane 1: U251 cell lysate
Lane 2: Mouse lung lysate
Lane 3: Rat brain lysate

Referencias

Datasheet

Anti-VCP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo