Anti-VCP Polyclonal Antibody (HX922014)

Name: Anti-VCP Polyclonal Antibody
Brand: abinScience
SKU: HX922014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HX922014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human VCP (Gly125-Ile371).

Cible

Transitional endoplasmic reticulum ATPase, Valosin-containing protein, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, VCP

Purification

Purified by antigen affinity column.

Numéro d'accès

P55072

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Transitional endoplasmic reticulum ATPase (VCP) is a ~89 kDa protein. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome.

1. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139

2. Kadowaki, H. et al. (2015) Cell reports 13, 944-56. PMID: 26565908

3. Turakhiya, A. et al. (2018) Molecular cell 70, 906-919.e7. PMID: 29804830

4. Gwon, Y. et al. (2021) Science (New York, N.Y.) 372, eabf6548. PMID: 34739333

5. Meerang, M. et al. (2011) Nature cell biology 13, 1376-82. PMID: 22020440

6. Acs, K. et al. (2011) Nature structural & molecular biology 18, 1345-50. PMID: 22120668

7. Mosbech, A. et al. (2012) Nature structural & molecular biology 19, 1084-92. PMID: 23042605

8. Davis, EJ. et al. (2012) Nature structural & molecular biology 19, 1093-100. PMID: 23042607

9. Fielden, J. et al. (2020) Nature communications 11, 1274. PMID: 32152270

10. Krastev, DB. et al. (2022) Nature cell biology 24, 62-73. PMID: 35013556

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-VCP polyclonal antibody at 1ug/mL on various samples.
Lane 1: U251 cell lysate
Lane 2: Mouse lung lysate
Lane 3: Rat brain lysate

Reférences

Fiche technique

Anti-VCP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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