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| カタログ番号 | HX922014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human VCP (Gly125-Ile371). | ||||||||
| ターゲット | Transitional endoplasmic reticulum ATPase, Valosin-containing protein, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, VCP | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P55072 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Transitional endoplasmic reticulum ATPase (VCP) is a ~89 kDa protein. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. 1. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139 2. Kadowaki, H. et al. (2015) Cell reports 13, 944-56. PMID: 26565908 3. Turakhiya, A. et al. (2018) Molecular cell 70, 906-919.e7. PMID: 29804830 4. Gwon, Y. et al. (2021) Science (New York, N.Y.) 372, eabf6548. PMID: 34739333 5. Meerang, M. et al. (2011) Nature cell biology 13, 1376-82. PMID: 22020440 6. Acs, K. et al. (2011) Nature structural & molecular biology 18, 1345-50. PMID: 22120668 7. Mosbech, A. et al. (2012) Nature structural & molecular biology 19, 1084-92. PMID: 23042605 8. Davis, EJ. et al. (2012) Nature structural & molecular biology 19, 1093-100. PMID: 23042607 9. Fielden, J. et al. (2020) Nature communications 11, 1274. PMID: 32152270 10. Krastev, DB. et al. (2022) Nature cell biology 24, 62-73. PMID: 35013556 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-VCP polyclonal antibody at 1ug/mL on various samples.
Lane 1: U251 cell lysate
Lane 2: Mouse lung lysate
Lane 3: Rat brain lysate
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