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Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16) (VK039033)

Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16)
Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16)
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Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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  • Datasheet

  • SDS

Vista general
Número de catálogoVK039033
Reactividad de especiesVenezuelan equine encephalitis virus (VEEV)
AplicacionesELISA, Neutralization
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG
ID de clonSKV16
Objetivo E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p130, p62, pE2
Nivel de endotoxinas < 10 EU/mg
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P09592
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Structural polyprotein (Spike glycoprotein) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Garmashova, N. et al. (2007) Journal of virology 81, 2472-84. PMID: 17108023
2. Garmashova, N. et al. (2007) Journal of virology 81, 13552-65. PMID: 17913819
3. Atasheva, S. et al. (2010) Journal of virology 84, 4158-71. PMID: 20147401
4. Carey, BD. et al. (2018) Journal of virology 92. PMID: 29769351
Nota For research use only
Imágenes
  • Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16)

    SDS-PAGE

    SDS-PAGE for Anti-VEEV Spike glycoprotein Antibody (SKV16)

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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