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Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16) (VK039033)

Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16)
Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерVK039033
Реактивность видовVenezuelan equine encephalitis virus (VEEV)
ПримененияELISA, Neutralization
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG
ID клонаSKV16
Цель E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p130, p62, pE2
Уровень эндотоксинов < 10 EU/mg
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P09592
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:2000-1:20000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Structural polyprotein (Spike glycoprotein) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Garmashova, N. et al. (2007) Journal of virology 81, 2472-84. PMID: 17108023
2. Garmashova, N. et al. (2007) Journal of virology 81, 13552-65. PMID: 17913819
3. Atasheva, S. et al. (2010) Journal of virology 84, 4158-71. PMID: 20147401
4. Carey, BD. et al. (2018) Journal of virology 92. PMID: 29769351
Примечание For research use only
Изображения
  • Anti-VEEV Spike glycoprotein E2 Recombinant Antibody (SKV16)

    SDS-PAGE

    SDS-PAGE for Anti-VEEV Spike glycoprotein Antibody (SKV16)

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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