Anti-VEEV Spike glycoprotein E1 Polyclonal Antibody (VK039014)

Name: Anti-VEEV Spike glycoprotein E1 Polyclonal Antibody
Brand: abinScience
SKU: VK039014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

VK039014

Описание

Anti-VEEV Spike glycoprotein E1 Polyclonal Antibody (VK039014) is a rabbit polyclonal antibody detecting Spike glycoprotein E1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Venezuelan equine encephalitis virus (VEEV)

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant VEEV Structural polyprotein (Tyr813-Ser1229).

Цель

Structural polyprotein, p130, Capsid protein, EC:3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Purified by antigen affinity column.

Номер доступа

P09592

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Structural polyprotein (E1) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Garmashova, N. et al. (2007) Journal of virology 81, 2472-84. PMID: 17108023

2. Garmashova, N. et al. (2007) Journal of virology 81, 13552-65. PMID: 17913819

3. Atasheva, S. et al. (2010) Journal of virology 84, 4158-71. PMID: 20147401

4. Carey, BD. et al. (2018) Journal of virology 92. PMID: 29769351

Примечание

For research use only

Изображения

Western blot

Western blot analysis was performed using anti-E1 polyclonal antibody at 1ug/mL on recombinant VEEV Spike glycoprotein E1 (Catalog No: VK039012).

Ссылки

Даташит