中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | VK039024 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody (VK039024) is a rabbit polyclonal antibody detecting Spike glycoprotein E2 in ELISA, IHC, WB.
Highlights
|
||||||||
| 種反応性 | Venezuelan equine encephalitis virus (VEEV) | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant VEEV Structural polyprotein (Ser335-Pro679). | ||||||||
| ターゲット | 6K protein, Assembly protein E3, C, Capsid protein, Coat protein, E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p130, p62, pE2 | ||||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P09592 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Structural polyprotein (E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Garmashova, N. et al. (2007) Journal of virology 81, 2472-84. PMID: 17108023 2. Garmashova, N. et al. (2007) Journal of virology 81, 13552-65. PMID: 17913819 3. Atasheva, S. et al. (2010) Journal of virology 84, 4158-71. PMID: 20147401 4. Carey, BD. et al. (2018) Journal of virology 92. PMID: 29769351 | ||||||||
| 注意事項 | For research use only |
Western blot analysis was performed using anti-VEEV-E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: ATG22134 100ng
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
