Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody (VK039024)

Name: Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody
Brand: abinScience
SKU: VK039024
Price: 168 USD
Availability: InStock

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Спецификация:

50ug
100ug

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Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

VK039024

Описание

Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody (VK039024) is a rabbit polyclonal antibody detecting Spike glycoprotein E2 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Venezuelan equine encephalitis virus (VEEV)

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant VEEV Structural polyprotein (Ser335-Pro679).

Цель

6K protein, Assembly protein E3, C, Capsid protein, Coat protein, E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p130, p62, pE2

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Purified by antigen affinity column.

Номер доступа

P09592

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Structural polyprotein (E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Garmashova, N. et al. (2007) Journal of virology 81, 2472-84. PMID: 17108023

2. Garmashova, N. et al. (2007) Journal of virology 81, 13552-65. PMID: 17913819

3. Atasheva, S. et al. (2010) Journal of virology 84, 4158-71. PMID: 20147401

4. Carey, BD. et al. (2018) Journal of virology 92. PMID: 29769351

Примечание

For research use only

Изображения

Western blot

Western blot analysis was performed using anti-VEEV-E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: ATG22134 100ng

Ссылки

Даташит

Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа