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Anti-Vesicular stomatitis Indiana virus/VSIV NP/Nucleoprotein Recombinant Nanobody (SAA1125) (VK434053)

Anti-Vesicular stomatitis Indiana virus/VSIV NP/Nucleoprotein Recombinant Nanobody (SAA1125)
Anti-Vesicular stomatitis Indiana virus/VSIV NP/Nucleoprotein Recombinant Nanobody (SAA1125)
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概要
カタログ番号VK434053
説明
Anti-Vesicular stomatitis Indiana virus/VSIV NP/Nucleoprotein Nanobody (SAA1125) [SAA1125] (VK434053) is a alpaca monoclonal antibody detecting Nucleoprotein in ELISA.
種反応性Vesicular stomatitis Indiana virus (VSIV)
アプリケーションELISA
宿主種Alpaca
クローン性Monoclonal
アイソタイプVHH-8His-Cys-tag
クローンIDSAA1125
ターゲット Nucleoprotein, NP, Nucleocapsid protein, Protein N, N
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Purified by Nickel column.
アクセッション番号 P03521
形態 Liquid
保存バッファー 0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:2000-1:20000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Nucleoprotein (NP) is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.

1. Green, TJ. et al. (2006) Science (New York, N.Y.) 313, 357-60. PMID: 16778022
2. Ge, P. et al. (2010) Science (New York, N.Y.) 327, 689-93. PMID: 20133572
3. Gumpper, RH. et al. (2018) Journal of virology 92. PMID: 29437970
4. Jenni, S. et al. (2022) Nature communications 13, 4802. PMID: 35970826
5. Zhou, K. et al. (2022) Nature communications 13, 5980. PMID: 36216930
6. Green, TJ. et al. (2011) Journal of virology 85, 2714-22. PMID: 21177817
7. Si, Z. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2111948119. PMID: 35476516
注意事項 For research use only.
イメージ
  • Anti-Vesicular stomatitis Indiana virus/VSIV NP/Nucleoprotein Recombinant Nanobody (SAA1125)

    SDS-PAGE

    SDS-PAGE for Anti-Vesicular stomatitis Indiana virus/VSIV NP/Nucleoprotein Nanobody (SAA1125)

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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