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Anti-HAV VP2 Polyclonal Antibody (VK079014)

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Vista general
Número de catálogoVK079014
Descripción
Anti-VP2 Polyclonal Antibody (VK079014) is a rabbit polyclonal antibody detecting 1B VP2 mature peptide; 1B VP2 mature peptide in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHepatitis A virus (HAV)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant hav VP2 (Asp1-Gln222).
Objetivo 1B VP2 mature peptide; 1B VP2 mature peptide
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P08617, Q67825, Q9DWR1, Q02381, P31788, Q05794, P13901, P06442, A5LGW7, P14553, Q5Y944, Q8V0N6, A3FMB2, P06441
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Genome polyprotein (VP2) is a ~251 kDa protein. Capsid proteins VP1, VP2, and VP3 form a closed capsid enclosing the viral positive strand RNA genome. All these proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, VP2, and VP3, with a diameter of approximately 300 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes. The naked capsid interacts with the host receptor HAVCR1 to provide virion attachment to and probably entry into the target cell.

1. Wang, X. et al. (2015) Nature 517, 85-88. PMID: 25327248
2. Wang, X. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 770-775. PMID: 28074040
3. Silberstein, E. et al. (2001) Journal of virology 75, 717-25. PMID: 11134285
4. Costafreda, MI. et al. (2018) Journal of virology 92. PMID: 29437974
5. Feigelstock, D. et al. (1998) Journal of virology 72, 6621-8. PMID: 9658108
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-HAV VP2 Polyclonal Antibody

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