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Anti-VSV Nucleoprotein Polyclonal Antibody (VK422064)

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Vista general
Número de catálogoVK422064
Descripción
Anti-VSIV Nucleoprotein/NP Polyclonal Antibody (VK422064) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesVesicular stomatitis Indiana virus (VSIV)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant VSIV Nucleoprotein/NP (Ser2-Lys422).
Objetivo N, NP, Nucleocapsid protein, Nucleoprotein, Protein N
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P03521
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Nucleoprotein (NP) is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.

1. Green, TJ. et al. (2006) Science (New York, N.Y.) 313, 357-60. PMID: 16778022
2. Ge, P. et al. (2010) Science (New York, N.Y.) 327, 689-93. PMID: 20133572
3. Gumpper, RH. et al. (2018) Journal of virology 92. PMID: 29437970
4. Jenni, S. et al. (2022) Nature communications 13, 4802. PMID: 35970826
5. Zhou, K. et al. (2022) Nature communications 13, 5980. PMID: 36216930
6. Green, TJ. et al. (2011) Journal of virology 85, 2714-22. PMID: 21177817
7. Si, Z. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2111948119. PMID: 35476516
Nota For research use only
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-VSV Nucleoprotein Polyclonal Antibody

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