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Anti-VSV Nucleoprotein Polyclonal Antibody (VK422064)

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Aperçu
Numéro de catalogueVK422064
Description
Anti-VSIV Nucleoprotein/NP Polyclonal Antibody (VK422064) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesVesicular stomatitis Indiana virus (VSIV)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant VSIV Nucleoprotein/NP (Ser2-Lys422).
Cible N, NP, Nucleocapsid protein, Nucleoprotein, Protein N
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P03521
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Nucleoprotein (NP) is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases.

1. Green, TJ. et al. (2006) Science (New York, N.Y.) 313, 357-60. PMID: 16778022
2. Ge, P. et al. (2010) Science (New York, N.Y.) 327, 689-93. PMID: 20133572
3. Gumpper, RH. et al. (2018) Journal of virology 92. PMID: 29437970
4. Jenni, S. et al. (2022) Nature communications 13, 4802. PMID: 35970826
5. Zhou, K. et al. (2022) Nature communications 13, 5980. PMID: 36216930
6. Green, TJ. et al. (2011) Journal of virology 85, 2714-22. PMID: 21177817
7. Si, Z. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2111948119. PMID: 35476516
Note For research use only
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-VSV Nucleoprotein Polyclonal Antibody

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