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Anti-VSV RdRp/Protein L Polyclonal Antibody (VK422034)

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Vista general
Número de catálogoVK422034
Descripción
Anti-VSIV RdRp/Protein L Polyclonal Antibody (VK422034) is a rabbit polyclonal antibody detecting VSIV RdRp/Protein L in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesVesicular stomatitis Indiana virus (VSIV)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant VSIV RdRp/Protein L (Glu2-Gly188).
Objetivo polymerase, partial, polymerase, partial
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q8B0I0, Q8B0H5, Q8B0H0, Q98776, P03523
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

RNA-directed RNA polymerase L (RdRp/Protein L) is a ~240 kDa protein. Multifunctional enzyme responsible for RNA synthesis (replicase and transcriptase), cap addition, and cap methylation. Also performs the polyadenylation of subgenomic mRNAs by a stuttering mechanism at a slipery stop site present at the end of viral genes. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). L is packaged into virions during assembly and translocates to the 3' leader promoter to initiate transcription after entering the host cells. During transcription and replication of the genome, L does not bind the N-RNA complex directly, but is bridged by its non-catalytic cofactor P, which interacts with L and N oligomers simultaneously.

Nota For research use only
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-VSV RdRp/Protein L Polyclonal Antibody

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