Anti-WAS Polyclonal Antibody (HW679024)

Name: Anti-WAS Polyclonal Antibody
Brand: abinScience
SKU: HW679024
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HW679024

Description

Anti-WAS Polyclonal Antibody (HW679024) is a rabbit polyclonal antibody detecting WAS in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human WAS (Leu39-Gly251).

Cible

Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp, WAS, IMD2

Purification

Purified by antigen affinity column.

Numéro d'accès

P42768

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Actin nucleation-promoting factor WAS is a ~52 kDa protein. Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

1. Cory, GO. et al. (2002) The Journal of biological chemistry 277, 45115-21. PMID: 12235133

2. Cory, GO. et al. (2003) Molecular cell 11, 1229-39. PMID: 12769847

3. Chereau, D. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 16644-9. PMID: 16275905

4. Symons, M. et al. (1996) Cell 84, 723-34. PMID: 8625410

5. Ramesh, N. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 14671-6. PMID: 9405671

6. Cheng, HC. et al. (2008) Nature 454, 1009-13. PMID: 18650809

7. Taylor, MD. et al. (2010) Science translational medicine 2, 37ra44. PMID: 20574068

8. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-WAS polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: HL-60 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: THP-1 cell lysate