Anti-WAS Polyclonal Antibody (HW679024)

Name: Anti-WAS Polyclonal Antibody
Brand: abinScience
SKU: HW679024
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HW679024

説明

Anti-WAS Polyclonal Antibody (HW679024) is a rabbit polyclonal antibody detecting WAS in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human WAS (Leu39-Gly251).

ターゲット

Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp, WAS, IMD2

精製

Purified by antigen affinity column.

アクセッション番号

P42768

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Actin nucleation-promoting factor WAS is a ~52 kDa protein. Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

1. Cory, GO. et al. (2002) The Journal of biological chemistry 277, 45115-21. PMID: 12235133

2. Cory, GO. et al. (2003) Molecular cell 11, 1229-39. PMID: 12769847

3. Chereau, D. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 16644-9. PMID: 16275905

4. Symons, M. et al. (1996) Cell 84, 723-34. PMID: 8625410

5. Ramesh, N. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 14671-6. PMID: 9405671

6. Cheng, HC. et al. (2008) Nature 454, 1009-13. PMID: 18650809

7. Taylor, MD. et al. (2010) Science translational medicine 2, 37ra44. PMID: 20574068

8. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-WAS polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: HL-60 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: THP-1 cell lysate