Anti-WAS Polyclonal Antibody (HW679024)

Name: Anti-WAS Polyclonal Antibody
Brand: abinScience
SKU: HW679024
Price: 96 USD
Availability: InStock

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Каталожный номер

HW679024

Описание

Anti-WAS Polyclonal Antibody (HW679024) is a rabbit polyclonal antibody detecting WAS in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human WAS (Leu39-Gly251).

Цель

Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp, WAS, IMD2

Очистка

Purified by antigen affinity column.

Номер доступа

P42768

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Actin nucleation-promoting factor WAS is a ~52 kDa protein. Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

1. Cory, GO. et al. (2002) The Journal of biological chemistry 277, 45115-21. PMID: 12235133

2. Cory, GO. et al. (2003) Molecular cell 11, 1229-39. PMID: 12769847

3. Chereau, D. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 16644-9. PMID: 16275905

4. Symons, M. et al. (1996) Cell 84, 723-34. PMID: 8625410

5. Ramesh, N. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 14671-6. PMID: 9405671

6. Cheng, HC. et al. (2008) Nature 454, 1009-13. PMID: 18650809

7. Taylor, MD. et al. (2010) Science translational medicine 2, 37ra44. PMID: 20574068

8. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-WAS polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: HL-60 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: THP-1 cell lysate