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| カタログ番号 | HV814014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human XPNPEP3 (Glu253-Gln482). | ||||||||
| ターゲット | Xaa-Pro aminopeptidase 3, APP3, XPNPEP3, Aminopeptidase P3, X-Pro aminopeptidase 3 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9NQH7 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Xaa-Pro aminopeptidase 3 (XPNPEP3) is a ~57 kDa protein. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position. 1. Inoue, M. et al. (2015) Journal of cell science 128, 656-69. PMID: 25609706 2. Singh, R. et al. (2017) The Journal of biological chemistry 292, 10035-10047. PMID: 28476889 | ||||||||
| 注意事項 | For research use only. |
A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting XPNPEP3 (HV814014) at a concentration of 41.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of XPNPEP3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-XPNPEP3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC3 cell lysate
Lane 4: Mouse kidney lysate
Lane 5: Mouse heart lysate
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