Anti-XPNPEP3 Polyclonal Antibody (HV814014)

Name: Anti-XPNPEP3 Polyclonal Antibody
Brand: abinScience
SKU: HV814014
Price: 96 USD
Availability: InStock

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HV814014

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human XPNPEP3 (Glu253-Gln482).

Цель

Xaa-Pro aminopeptidase 3, APP3, XPNPEP3, Aminopeptidase P3, X-Pro aminopeptidase 3

Очистка

Purified by antigen affinity column.

Номер доступа

Q9NQH7

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Xaa-Pro aminopeptidase 3 (XPNPEP3) is a ~57 kDa protein. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position.

1. Inoue, M. et al. (2015) Journal of cell science 128, 656-69. PMID: 25609706

2. Singh, R. et al. (2017) The Journal of biological chemistry 292, 10035-10047. PMID: 28476889

Примечание

For research use only.

Изображения

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting XPNPEP3 (HV814014) at a concentration of 41.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of XPNPEP3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-XPNPEP3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC3 cell lysate
Lane 4: Mouse kidney lysate
Lane 5: Mouse heart lysate

Ссылки

Даташит

Anti-XPNPEP3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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