Anti-XRCC1 Polyclonal Antibody (HB640014)

Name: Anti-XRCC1 Polyclonal Antibody
Brand: abinScience
SKU: HB640014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HB640014

説明

Anti-XRCC1 Polyclonal Antibody (HB640014) is a rabbit polyclonal antibody detecting XRCC1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

種反応性

Human

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human XRCC1 (Thr284-Ala633).

ターゲット

DNA repair protein XRCC1, XRCC1, X-ray repair cross-complementing protein 1

精製

Purified by antigen affinity column.

アクセッション番号

P18887

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA repair protein XRCC1 is a ~69 kDa protein. Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.

1. Whitehouse, CJ. et al. (2001) Cell 104, 107-17. PMID: 11163244

2. Hoch, NC. et al. (2017) Nature 541, 87-91. PMID: 28002403

3. Demin, AA. et al. (2021) Molecular cell 81, 3018-3030.e5. PMID: 34102106

4. Adamowicz, M. et al. (2021) Nature cell biology 23, 1287-1298. PMID: 34811483

5. El-Khamisy, SF. et al. (2003) Nucleic acids research 31, 5526-33. PMID: 14500814

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-XRCC1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse testis lysate

参考文献

データシート

Anti-XRCC1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-XRCC1 Polyclonal Antibody (HB640014)

概要

イメージ

参考文献

データシート

SDS