Anti-XRCC5/Ku80 Polyclonal Antibody (HY071014)

Name: Anti-XRCC5/Ku80 Polyclonal Antibody
Brand: abinScience
SKU: HY071014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Referencias
Datasheet
SDS

Vista general

Número de catálogo

HY071014

Descripción

Anti-XRCC5/Ku80 Polyclonal Antibody (HY071014) is a rabbit polyclonal antibody detecting XRCC5 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human XRCC5/Ku80 (Leu384-Ile732).

Objetivo

Lupus Ku autoantigen protein p86, XRCC5, Thyroid-lupus autoantigen, Ku86, ATP-dependent DNA helicase 2 subunit 2, CTC85, G22P2, DNA repair protein XRCC5, 86 kDa subunit of Ku antigen, TLAA, Nuclear factor IV, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit, CTCBF, Ku80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5

Purificación

Purified by antigen affinity column.

Número de acceso

P13010

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

DNA repair protein Ku80 (XRCC5) is a ~82 kDa protein. DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC6 (Ku70), creating the Ku70:Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends. This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination. Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs.

1. Walker, JR. et al. (2001) Nature 412, 607-14. PMID: 11493912

2. Chen, S. et al. (2021) Nature 593, 294-298. PMID: 33854234

3. Chaplin, AK. et al. (2021) Molecular cell 81, 3400-3409.e3. PMID: 34352203

4. West, RB. et al. (1998) Molecular and cellular biology 18, 5908-20. PMID: 9742108

5. Roberts, SA. et al. (2010) Nature 464, 1214-7. PMID: 20383123

6. Tuteja, N. et al. (1994) The EMBO journal 13, 4991-5001. PMID: 7957065

7. Maekawa, T. et al. (2018) Nucleic acids research 46, 4487-4504. PMID: 29490055

8. Willis, DM. et al. (2002) The Journal of biological chemistry 277, 37280-91. PMID: 12145306

9. Chung, U. et al. (1996) The Journal of biological chemistry 271, 8593-8. PMID: 8621488

10. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-XRCC5/Ku80 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo