中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HY071014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-XRCC5/Ku80 Polyclonal Antibody (HY071014) is a rabbit polyclonal antibody detecting XRCC5 in ELISA, IHC, WB. Suitable for Human.
Highlights
|
||||||||
| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human XRCC5/Ku80 (Leu384-Ile732). | ||||||||
| ターゲット | Lupus Ku autoantigen protein p86, XRCC5, Thyroid-lupus autoantigen, Ku86, ATP-dependent DNA helicase 2 subunit 2, CTC85, G22P2, DNA repair protein XRCC5, 86 kDa subunit of Ku antigen, TLAA, Nuclear factor IV, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit, CTCBF, Ku80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P13010 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | DNA repair protein Ku80 (XRCC5) is a ~82 kDa protein. DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC6 (Ku70), creating the Ku70:Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends. This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination. Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs. 1. Walker, JR. et al. (2001) Nature 412, 607-14. PMID: 11493912 2. Chen, S. et al. (2021) Nature 593, 294-298. PMID: 33854234 3. Chaplin, AK. et al. (2021) Molecular cell 81, 3400-3409.e3. PMID: 34352203 4. West, RB. et al. (1998) Molecular and cellular biology 18, 5908-20. PMID: 9742108 5. Roberts, SA. et al. (2010) Nature 464, 1214-7. PMID: 20383123 6. Tuteja, N. et al. (1994) The EMBO journal 13, 4991-5001. PMID: 7957065 7. Maekawa, T. et al. (2018) Nucleic acids research 46, 4487-4504. PMID: 29490055 8. Willis, DM. et al. (2002) The Journal of biological chemistry 277, 37280-91. PMID: 12145306 9. Chung, U. et al. (1996) The Journal of biological chemistry 271, 8593-8. PMID: 8621488 10. Morchikh, M. et al. (2017) Molecular cell 67, 387-399.e5. PMID: 28712728 | ||||||||
| 注意事項 | For research use only. |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
