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Anti-YTHDF2 Polyclonal Antibody (MP332014)

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Aperçu
Numéro de catalogueMP332014
Description
Anti-YTHDF2 Polyclonal Antibody (MP332014) is a rabbit polyclonal antibody detecting YTH domain-containing family protein 2; Ythdf2 in ELISA, IHC, WB. Suitable for Bovine, Mouse, Zebrafish, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant mouse YTHDF2 (Pro385-Lys579).
Cible YTH domain-containing family protein 2; Ythdf2
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès E7F1H9, Q7Z739, Q9Y5A9, Q8BYK6, Q9BYJ9, Q0VCZ3, Q91YT7, P59326
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

YTH domain-containing family protein 2 (YTHDF2) is a ~66 kDa protein. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs. The YTHDF paralogs (ythdf1, ythdf2 and ythdf3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. Plays a key role in maternal-to-zygotic transition during early embryonic development, the process during which maternally inherited mRNAs are degraded: acts by binding m6A-containing maternal mRNAs and promoting their degradation.

1. Zhao, BS. et al. (2017) Nature 542, 475-478. PMID: 28192787
2. Zhang, C. et al. (2017) Nature 549, 273-276. PMID: 28869969
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Volume
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-YTHDF2 Polyclonal Antibody

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