Anti-YWHAZ Polyclonal Antibody (HX859014)

Name: Anti-YWHAZ Polyclonal Antibody
Brand: abinScience
SKU: HX859014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Número de catálogo

HX859014

Descripción

Anti-YWHAZ Polyclonal Antibody (HX859014) is a rabbit polyclonal antibody detecting YWHAZ in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human YWHAZ (Met1-Asn245).

Objetivo

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

Purificación

Purified by antigen affinity column.

Número de acceso

P63104

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

14-3-3 protein zeta/delta (YWHAZ) is a ~27 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation.

1. Zheng, W. et al. (2003) Nature structural biology 10, 1054-7. PMID: 14578935

2. Tsuruta, F. et al. (2004) The EMBO journal 23, 1889-99. PMID: 15071501

3. Ganguly, S. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1222-7. PMID: 15644438

4. Gu, YM. et al. (2006) FEBS letters 580, 305-10. PMID: 16376338

5. Angrand, PO. et al. (2006) Molecular & cellular proteomics : MCP 5, 2211-27. PMID: 16959763

6. Popov, IK. et al. (2019) Frontiers in physiology 10, 388. PMID: 31024343

7. Dubois, T. et al. (1997) The Journal of biological chemistry 272, 28882-8. PMID: 9360956

8. Zhang, Z. et al. (2022) Molecular cell 82, 2844-2857.e10. PMID: 35662396

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-YWHAZ polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: Raji cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Hela cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Mouse kidney lysate
Lane11: Rat brain lysate
Lane12: Rat kidney lysate

Referencias

Datasheet

Anti-YWHAZ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-YWHAZ Polyclonal Antibody (HX859014)

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Imágenes

Referencias

Datasheet

SDS