Anti-YWHAZ Polyclonal Antibody (HX859014)

Name: Anti-YWHAZ Polyclonal Antibody
Brand: abinScience
SKU: HX859014
Price: 96 USD
Availability: InStock

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50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HX859014

Description

Anti-YWHAZ Polyclonal Antibody (HX859014) is a rabbit polyclonal antibody detecting YWHAZ in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human YWHAZ (Met1-Asn245).

Cible

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

Purification

Purified by antigen affinity column.

Numéro d'accès

P63104

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

14-3-3 protein zeta/delta (YWHAZ) is a ~27 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation.

1. Zheng, W. et al. (2003) Nature structural biology 10, 1054-7. PMID: 14578935

2. Tsuruta, F. et al. (2004) The EMBO journal 23, 1889-99. PMID: 15071501

3. Ganguly, S. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1222-7. PMID: 15644438

4. Gu, YM. et al. (2006) FEBS letters 580, 305-10. PMID: 16376338

5. Angrand, PO. et al. (2006) Molecular & cellular proteomics : MCP 5, 2211-27. PMID: 16959763

6. Popov, IK. et al. (2019) Frontiers in physiology 10, 388. PMID: 31024343

7. Dubois, T. et al. (1997) The Journal of biological chemistry 272, 28882-8. PMID: 9360956

8. Zhang, Z. et al. (2022) Molecular cell 82, 2844-2857.e10. PMID: 35662396

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-YWHAZ polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: Raji cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Hela cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Mouse kidney lysate
Lane11: Rat brain lysate
Lane12: Rat kidney lysate

Reférences

Fiche technique

Anti-YWHAZ Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-YWHAZ Polyclonal Antibody (HX859014)

Aperçu

Images

Reférences

Fiche technique

SDS