Anti-YWHAZ Polyclonal Antibody (HX859014)

Name: Anti-YWHAZ Polyclonal Antibody
Brand: abinScience
SKU: HX859014
Price: 96 USD
Availability: InStock

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Каталожный номер

HX859014

Описание

Anti-YWHAZ Polyclonal Antibody (HX859014) is a rabbit polyclonal antibody detecting YWHAZ in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human YWHAZ (Met1-Asn245).

Цель

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

Очистка

Purified by antigen affinity column.

Номер доступа

P63104

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

14-3-3 protein zeta/delta (YWHAZ) is a ~27 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation.

1. Zheng, W. et al. (2003) Nature structural biology 10, 1054-7. PMID: 14578935

2. Tsuruta, F. et al. (2004) The EMBO journal 23, 1889-99. PMID: 15071501

3. Ganguly, S. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1222-7. PMID: 15644438

4. Gu, YM. et al. (2006) FEBS letters 580, 305-10. PMID: 16376338

5. Angrand, PO. et al. (2006) Molecular & cellular proteomics : MCP 5, 2211-27. PMID: 16959763

6. Popov, IK. et al. (2019) Frontiers in physiology 10, 388. PMID: 31024343

7. Dubois, T. et al. (1997) The Journal of biological chemistry 272, 28882-8. PMID: 9360956

8. Zhang, Z. et al. (2022) Molecular cell 82, 2844-2857.e10. PMID: 35662396

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-YWHAZ polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: Raji cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Hela cell lysate
Lane 8: PC-12 cell lysate
Lane 9: Mouse brain lysate
Lane 10: Mouse kidney lysate
Lane11: Rat brain lysate
Lane12: Rat kidney lysate

Ссылки

Даташит

Anti-YWHAZ Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-YWHAZ Polyclonal Antibody (HX859014)

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