Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody (VK512014)

Name: Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody
Brand: abinScience
SKU: VK512014
Price: 168 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

VK512014

Reactividad de especies

Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Zaire ebolavirus NP/Nucleo(Val36-Glu351).

Objetivo

Nucleoprotein, Nucleocapsid protein, NP

Purificación

Purified by antigen affinity column.

Número de acceso

O72142, P18272

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-Ebola virus Nucleoprotein polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 70 ug
Lane 2: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 50 ug
Lane 3: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 30 ug
Lane 4: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 10 ug

Referencias

Datasheet

Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo