

| Каталожный номер | VK512014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Реактивность видов | Zaire ebolavirus (ZEBOV) (Zaire Ebola virus) | ||||||||
| Применения | ELISA, IHC, WB | ||||||||
| Хозяинский вид | Rabbit | ||||||||
| Клоональность | Polyclonal | ||||||||
| Изотип | IgG | ||||||||
| Иммуноген | E. coli - derived recombinant Zaire ebolavirus NP/Nucleo(Val36-Glu351). | ||||||||
| Цель | Nucleoprotein, Nucleocapsid protein, NP | ||||||||
| Очистка | Purified by antigen affinity column. | ||||||||
| Номер доступа | O72142, P18272 | ||||||||
| Форма | Liquid | ||||||||
| Буфер хранения | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
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| Информация об использовании продукта |
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| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Фон | Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm. | ||||||||
| Примечание | For research use only. |

Western blot analysis was performed using anti-Ebola virus Nucleoprotein polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 70 ug
Lane 2: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 50 ug
Lane 3: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 30 ug
Lane 4: recombinant Zaire ebolavirus NP/Nucleoprotein (Catalog No: VK512012), 10 ug
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