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Anti-ZAP70 Polyclonal Antibody (HW782014)

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Anti-ZAP70 Polyclonal Antibody
Anti-ZAP70 Polyclonal Antibody
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Especificación:
  • 50ug
  • 100ug
Número:
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Vista general
Número de catálogoHW782014
Descripción
Anti-ZAP70 Polyclonal Antibody (HW782014) is a rabbit polyclonal antibody detecting ZAP70 in ELISA, IHC, WB. Suitable for Mouse and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human ZAP70 (Leu337-Leu600).
Objetivo Tyrosine-protein kinase ZAP-70, ZAP70, Syk-related tyrosine kinase, 70 kDa zeta-chain associated protein, SRK
Purificación Purified by antigen affinity column.
Número de acceso P43403
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Tyrosine-protein kinase ZAP-70 (ZAP70) is a ~69 kDa protein. Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Also contributes to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the phosphorylated TCR components CD3E and CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation.

1. Iwashima, M. et al. (1994) Science (New York, N.Y.) 263, 1136-9. PMID: 7509083
Nota For research use only.
Imágenes
  • Anti-ZAP70 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ZAP70 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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