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Anti-ZEBOV VP35 Polyclonal Antibody (VK661014)

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Número de catálogoVK661014
Descripción
Anti-ZEBOV VP35 Polyclonal Antibody (VK661014) is a rabbit polyclonal antibody detecting VP35 in ELISA, IHC, WB. Suitable for Reston ebolavirus, Zaire ebolavirus, and Sudan ebolavirus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesZaire ebolavirus (ZEBOV) (Zaire Ebola virus)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant ZEBOV VP35 (Gly215-Ile340).
Objetivo Polymerase cofactor VP35, Ebola VP35, eVP35, VP35
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q8JPY0, Q5XX07, Q05127, Q6V1Q9, Q91DE0
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Polymerase cofactor VP35 is a ~36 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1.

1. Kimberlin, CR. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 314-9. PMID: 20018665
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ZEBOV VP35 Polyclonal Antibody

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