Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9) (VK661013)

Name: Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9)
Brand: abinScience
SKU: VK661013
Price: 191 USD
Availability: InStock

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Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

VK661013

Reactividad de especies

Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)

Aplicaciones

ELISA, WB

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG

ID de clon

Objetivo

VP35, Polymerase cofactor VP35, Ebola VP35, eVP35

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q05127

RRID

Anti-ZEBOV VP35/Polymerase cofactor VP35 Antibody (F9) (abinScience Cat# VK661013, RRID:AB_3727248)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Polymerase cofactor VP35 is a ~37 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1.

1. Basler, CF. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 12289-94. PMID: 11027311

2. van Tol, S. et al. (2022) PLoS pathogens 18, e1010532. PMID: 35533195

3. Enterlein, S. et al. (2006) Antimicrobial agents and chemotherapy 50, 984-93. PMID: 16495261

4. Brown, CS. et al. (2014) Journal of molecular biology 426, 2045-58. PMID: 24495995

5. Mühlberger, E. et al. (1999) Journal of virology 73, 2333-42. PMID: 9971816

6. Watanabe, R. et al. (2024) Cell 187, 5587-5603.e19. PMID: 39293445

7. Luthra, P. et al. (2015) Journal of virology 89, 5148-53. PMID: 25741013

8. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894

9. Yen, BC. et al. (2016) Journal of virology 90, 5108-5118. PMID: 26962215

10. Luthra, P. et al. (2013) Cell host & microbe 14, 74-84. PMID: 23870315

Nota

For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Anti-ZEBOV VP35/Polymerase cofactor VP35 Antibody (F9)

Referencias

Datasheet

Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo