Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9) (VK661013)

Name: Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9)
Brand: abinScience
SKU: VK661013
Price: 191 USD
Availability: InStock

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Prix(USD) :

Spécification :

50ug
100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

VK661013

Reactivité des espèces

Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)

Applications

ELISA, WB

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG

ID de clone

Cible

VP35, Polymerase cofactor VP35, Ebola VP35, eVP35

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q05127

RRID

Anti-ZEBOV VP35/Polymerase cofactor VP35 Antibody (F9) (abinScience Cat# VK661013, RRID:AB_3727248)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

Polymerase cofactor VP35 is a ~37 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1.

1. Basler, CF. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 12289-94. PMID: 11027311

2. van Tol, S. et al. (2022) PLoS pathogens 18, e1010532. PMID: 35533195

3. Enterlein, S. et al. (2006) Antimicrobial agents and chemotherapy 50, 984-93. PMID: 16495261

4. Brown, CS. et al. (2014) Journal of molecular biology 426, 2045-58. PMID: 24495995

5. Mühlberger, E. et al. (1999) Journal of virology 73, 2333-42. PMID: 9971816

6. Watanabe, R. et al. (2024) Cell 187, 5587-5603.e19. PMID: 39293445

7. Luthra, P. et al. (2015) Journal of virology 89, 5148-53. PMID: 25741013

8. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894

9. Yen, BC. et al. (2016) Journal of virology 90, 5108-5118. PMID: 26962215

10. Luthra, P. et al. (2013) Cell host & microbe 14, 74-84. PMID: 23870315

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-ZEBOV VP35/Polymerase cofactor VP35 Antibody (F9)

Reférences

Fiche technique

Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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