HEK293T Human CD184/CXCR4 Stable Cell Line (HX206818)

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カタログ番号	HX206818
説明	HEK293T Human CD184/CXCR4 Stable Cell Line (HX206818) is a stable cell line expressing CD184/CXCR4. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
アクセッション番号	P61073
アプリケーション	FCM
宿主種	HEK293T
種	Human
ターゲット	CD184/CXCR4
検出方法	Colorimetric
サンプルタイプ	Plasma, Serum
アッセイタイプ	Quantitative
レンジ	1.56 - 100 ng/mL
感度	0.25 ng/mL
回収率	80-120%
特性	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
発送	2-8 ℃
安定性と保存	Frozen in liquid nitrogen.
仕様	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景	C-X-C chemokine receptor type 4 (CD184/CXCR4) is a ~39 kDa protein. Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. CXCR4 is coupled to G(i) G alpha proteins and mediates inhibition of adenylate cyclase. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. 1. Brelot, A. et al. (1999) Journal of virology 73, 2576-86. PMID: 10074102 2. Gupta, SK. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 2368-72. PMID: 10452968 3. Cheng, ZJ. et al. (2000) The Journal of biological chemistry 275, 2479-85. PMID: 10644702 4. Brelot, A. et al. (2000) The Journal of biological chemistry 275, 23736-44. PMID: 10825158 5. Veldkamp, CT. et al. (2008) Science signaling 1, ra4. PMID: 18799424 6. Busillo, JM. et al. (2010) The Journal of biological chemistry 285, 7805-17. PMID: 20048153 7. Malik, R. et al. (2010) Molecular biology of the cell 21, 2529-41. PMID: 20505072 8. Cao, Y. et al. (2015) Leukemia 29, 169-76. PMID: 24912431 9. Lear, T. et al. (2017) American journal of physiology. Cell physiology 313, C584-C592. PMID: 28978524 10. Bleul, CC. et al. (1996) Nature 382, 829-33. PMID: 8752280
注意事項	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CXCR4 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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