HEK293T Human CD20/MS4A1 Stable Cell Line (HY257818)

カタログ番号	HY257818
説明	HEK293T Human CD20/MS4A1 Stable Cell Line (HY257818) is a stable cell line expressing CD20/MS4A1. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
アクセッション番号	P11836
アプリケーション	FCM
宿主種	HEK293T
種	Human
ターゲット	CD20/MS4A1
特性	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
安定性と保存	Frozen in liquid nitrogen.
仕様	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景	B-lymphocyte antigen CD20 (MS4A1) is a ~33 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva). 1. Li, H. et al. (2003) The Journal of biological chemistry 278, 42427-34. PMID: 12920111 2. Tedder, TF. et al. (1985) Journal of immunology (Baltimore, Md. : 1950) 135, 973-9. PMID: 3925015 3. Bubien, JK. et al. (1993) The Journal of cell biology 121, 1121-32. PMID: 7684739 4. Polyak, MJ. et al. (2008) The Journal of biological chemistry 283, 18545-52. PMID: 18474602

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD20 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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