HEK293T Human CD324/CDH1 Stable Cell Line (HY460818)

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Numéro de catalogue	HY460818
Description	HEK293T Human CD324/CDH1 Stable Cell Line (HY460818) is a stable cell line expressing CD324/CDH1. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Numéro d'accès	P12830
Applications	FCM
Espèce hôte	HEK293T
Espèces	Human
Cible	CD324/CDH1
Propriétés	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stabilité et stockage	Frozen in liquid nitrogen.
Spécifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Contexte	Cadherin-1 (CD324/CDH1) is a ~97 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. 1. Meigs, TE. et al. (2002) The Journal of biological chemistry 277, 24594-600. PMID: 11976333 2. Shafraz, O. et al. (2018) eLife 7. PMID: 29999492

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CDH1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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