HEK293T Human EGFRvIII Stable Cell Line (HF004818)

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Numéro de catalogue	HF004818
Description	HEK293T Human EGFRvIII Stable Cell Line (HF004818) is a stable cell line expressing EGFRvIII. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Numéro d'accès	P00533
Applications	FCM
Espèce hôte	HEK293T
Espèces	Human
Cible	EGFRvIII
Propriétés	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stabilité et stockage	Frozen in liquid nitrogen.
Spécifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Contexte	Epidermal growth factor receptor (EGFRvIII) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. 1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725 2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536 3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960 4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033 5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049 6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079 7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029 8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human EGFRVIII monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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