HEK293T Human FAP Stable Cell Line (HC440818)

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カタログ番号	HC440818
説明	HEK293T Human FAP Stable Cell Line (HC440818) is a stable cell line expressing FAP. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
アクセッション番号	Q12884
アプリケーション	FCM
宿主種	HEK293T
種	Human
ターゲット	FAP
特性	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
安定性と保存	Frozen in liquid nitrogen.
仕様	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
背景	Prolyl endopeptidase FAP is a ~87 kDa protein. Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein. Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). 1. Lee, KN. et al. (2004) Blood 103, 3783-8. PMID: 14751930 2. Lee, KN. et al. (2006) Blood 107, 1397-404. PMID: 16223769 3. Edosada, CY. et al. (2006) The Journal of biological chemistry 281, 7437-44. PMID: 16410248 4. Edosada, CY. et al. (2006) FEBS letters 580, 1581-6. PMID: 16480718 5. Meadows, SA. et al. (2007) Biochemistry 46, 4598-605. PMID: 17381073 6. Aggarwal, S. et al. (2008) Biochemistry 47, 1076-86. PMID: 18095711 7. Huang, CH. et al. (2011) Journal of biochemistry 149, 685-92. PMID: 21288888 8. Keane, FM. et al. (2013) FEBS open bio 4, 43-54. PMID: 24371721 9. Levy, MT. et al. (1999) Hepatology (Baltimore, Md.) 29, 1768-78. PMID: 10347120 10. Mueller, SC. et al. (1999) The Journal of biological chemistry 274, 24947-52. PMID: 10455171

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human 5T4 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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