InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12) (MS870209)

Name: InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12)
Brand: abinScience
SKU: MS870209
Price: 128 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

1mg
5mg
25mg
50mg
100mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	MS870209
Descripción	InVivo Plus Anti-Mouse CD279/PDCD1/PD1 Antibody (29F.1A12) [29F.1A12] (MS870209) is a rat monoclonal antibody detecting Programmed cell death protein 1 in FCM, IF, IHC, Neutralization, WB. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Reactividad de especies	Mouse
Aplicaciones	FCM, IF, IHC, Neutralization, WB
Especie huésped	Rat
Clonalidad	Monoclonal
Isotipo	IgG2a, kappa
ID de clon	29F.1A12
Objetivo	CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, PD-1
Nivel de endotoxinas	< 1 EU/mg
Pureza	>95% as determined by SDS-PAGE.
Productos asociados	pH 7.4 Dilution Buffer
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	Q02242
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo	Programmed cell death protein 1 (CD279/PD-1) is a ~31 kDa protein. Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. CD279 is the therapeutic target of pembrolizumab (Keytruda) and nivolumab (Opdivo). 1. Nishimura, H. et al. (1999) Immunity 11, 141-51. PMID: 10485649 2. Nishimura, H. et al. (2001) Science (New York, N.Y.) 291, 319-22. PMID: 11209085 3. Okazaki, T. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13866-71. PMID: 11698646 4. Okazaki, T. et al. (2011) The Journal of experimental medicine 208, 395-407. PMID: 21300912 5. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 6. Latchman, Y. et al. (2001) Nature immunology 2, 261-8. PMID: 11224527 7. Lin, DY. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3011-6. PMID: 18287011 8. Lázár-Molnár, E. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 10483-8. PMID: 18641123 9. Yokosuka, T. et al. (2012) The Journal of experimental medicine 209, 1201-17. PMID: 22641383
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12)
Bioactivity

Mice treatment with 29F.1A12 significantly reduced tumor growth compared with the Rat IgG2a isotype control (RF533109), as shown by lower tumor volumes during the observation period.
Bioactivity

Detects mouse PD1 (MS870011) in indirect ELISA.

Referencias

Datasheet

InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo