InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12) (MS870209)

Name: InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12)
Brand: abinScience
SKU: MS870209
Price: 128 USD
Availability: InStock

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Aperçu

Numéro de catalogue	MS870209
Description	InVivo Plus Anti-Mouse CD279/PDCD1/PD1 Antibody (29F.1A12) [29F.1A12] (MS870209) is a rat monoclonal antibody detecting Programmed cell death protein 1 in FCM, IF, IHC, Neutralization, WB. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Reactivité des espèces	Mouse
Applications	FCM, IF, IHC, Neutralization, WB
Espèce hôte	Rat
Clonalité	Monoclonal
Isotype	IgG2a, kappa
ID de clone	29F.1A12
Cible	CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, PD-1
Niveau d'endotoxines	< 1 EU/mg
Pureté	>95% as determined by SDS-PAGE.
Produits associés	pH 7.4 Dilution Buffer
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	Q02242
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	Programmed cell death protein 1 (CD279/PD-1) is a ~31 kDa protein. Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. CD279 is the therapeutic target of pembrolizumab (Keytruda) and nivolumab (Opdivo). 1. Nishimura, H. et al. (1999) Immunity 11, 141-51. PMID: 10485649 2. Nishimura, H. et al. (2001) Science (New York, N.Y.) 291, 319-22. PMID: 11209085 3. Okazaki, T. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13866-71. PMID: 11698646 4. Okazaki, T. et al. (2011) The Journal of experimental medicine 208, 395-407. PMID: 21300912 5. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 6. Latchman, Y. et al. (2001) Nature immunology 2, 261-8. PMID: 11224527 7. Lin, DY. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3011-6. PMID: 18287011 8. Lázár-Molnár, E. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 10483-8. PMID: 18641123 9. Yokosuka, T. et al. (2012) The Journal of experimental medicine 209, 1201-17. PMID: 22641383
Note	For research use only.

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SDS-PAGE

SDS-PAGE for InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12)
Bioactivity

Mice treatment with 29F.1A12 significantly reduced tumor growth compared with the Rat IgG2a isotype control (RF533109), as shown by lower tumor volumes during the observation period.
Bioactivity

Detects mouse PD1 (MS870011) in indirect ELISA.

Reférences

Fiche technique

InVivo Plus Anti-Mouse PD-1 Antibody(29F.1A12).pdf

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