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| カタログ番号 | HF739040 |
|---|---|
| 説明 |
InVivoMAb Anti-Human APOE2/3/4 Antibody (HJ15.4) [HJ15.4] (HF739040) is a mouse monoclonal antibody detecting Apo-E in ELISA, FuncS. Suitable for Human.
|
| 種反応性 | Human |
| アプリケーション | ELISA, FuncS |
| 宿主種 | Mouse |
| クローン性 | Monoclonal |
| アイソタイプ | IgG2a, kappa |
| クローンID | HJ15.4 |
| ターゲット | Apo-E, Apolipoprotein E, APOE |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 関連製品 | pH 7.4 Dilution Buffer |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P02649 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 背景 | Apolipoprotein E (APOE2/3) is a ~36 kDa protein. APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. 1. Krimbou, L. et al. (2004) Journal of lipid research 45, 839-48. PMID: 14754908 2. Arnon, R. et al. (1991) Biochimica et biophysica acta 1085, 336-42. PMID: 1911868 3. Marcel, YL. et al. (1983) Biochimica et biophysica acta 750, 411-7. PMID: 6860692 4. Sehayek, E. et al. (1991) The Journal of biological chemistry 266, 18259-67. PMID: 1917954 5. Verghese, PB. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E1807-16. PMID: 23620513 6. Kowal, RC. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 5810-4. PMID: 2762297 7. Ji, ZS. et al. (1997) The Journal of biological chemistry 272, 31285-92. PMID: 9395455 8. Li, X. et al. (2003) Biochemistry 42, 10406-17. PMID: 12950167 |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
Western blot analysis was performed using anti-APOE4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human APOE4 (Catalog No: HF739022)
Lane 2: negative control
Flow-cytometry using anti-human APOE antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human APOE monoclonal antibody (Catalog HF739040, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for InVivoMAb Anti-Human APOE2/3/4 Antibody (HJ15.4)
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