InVivoMAb Anti-Human Cannabinoid R1/CB1/CNR1 Antibody (5D12) (HB822010)

Name: InVivoMAb Anti-Human Cannabinoid R1/CB1/CNR1 Antibody (5D12)
Brand: abinScience
SKU: HB822010
Price: 370 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD):

スペック:

100ug
1mg

数量:

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HB822010

説明

InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12) [5D12] (HB822010) is a alpaca monoclonal antibody detecting CNR1 in ELISA, Neutralization. Suitable for Human.

種反応性

Human

アプリケーション

ELISA, Neutralization, WB

宿主種

Alpaca

クローン性

Monoclonal

アイソタイプ

VHH-8His-Cys-tag

クローンID

5D12

テスト済みアプリケーション

ターゲット

CNR1, CB1, CANN6, CB-R, CNR, Cannabinoid receptor 1

エンドトキシンレベル

<0.01 EU/ug

純度

>95% as determined by SDS-PAGE.

関連製品

pH 7.4 Dilution Buffer

精製

Purified by Nickel column.

アクセッション番号

P21554

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Cannabinoid receptor 1 (CNR1/CB1) is a ~52 kDa protein. G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta(9)-tetrahydrocannabinol (THC). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain. Signaling typically involves reduction in cyclic AMP. In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses.

1. Ryberg, E. et al. (2005) FEBS letters 579, 259-64. PMID: 15620723

2. Hua, T. et al. (2016) Cell 167, 750-762.e14. PMID: 27768894

3. Shao, Z. et al. (2016) Nature 540, 602-606. PMID: 27851727

4. Yang, X. et al. (2022) Nature chemical biology 18, 831-840. PMID: 35637350

5. Gérard, CM. et al. (1991) The Biochemical journal 279 ( Pt 1), 129-34. PMID: 1718258

6. Oddi, S. et al. (2012) British journal of pharmacology 165, 2635-51. PMID: 21895628

7. Niehaus, JL. et al. (2007) Molecular pharmacology 72, 1557-66. PMID: 17895407

8. Jourdan, T. et al. (2013) Nature medicine 19, 1132-40. PMID: 23955712

注意事項

For research use only. Not suitable for clinical or therapeutic use.

イメージ

SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12)
Bioactivity

Detects recombinant CNR1 (Catalog No: HB822012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CNR1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CNR1 (Catalog No: HB822012)
Immunofluorescence

Immunofluorescence staining of SHSY5Y with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane，Cytoplasm.