InVivoMAb Anti-Human Cannabinoid R1/CB1/CNR1 Antibody (5D12) (HB822010)

Name: InVivoMAb Anti-Human Cannabinoid R1/CB1/CNR1 Antibody (5D12)
Brand: abinScience
SKU: HB822010
Price: 370 USD
Availability: InStock

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Цена(USD):

Спецификация:

100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB822010

Описание

InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12) [5D12] (HB822010) is a alpaca monoclonal antibody detecting CNR1 in ELISA, Neutralization. Suitable for Human.

Реактивность видов

Human

Применения

ELISA, Neutralization, WB

Хозяинский вид

Alpaca

Клоональность

Monoclonal

Изотип

VHH-8His-Cys-tag

ID клона

5D12

Проверенные приложения

Цель

CNR1, CB1, CANN6, CB-R, CNR, Cannabinoid receptor 1

Уровень эндотоксинов

<0.01 EU/ug

Чистота

>95% as determined by SDS-PAGE.

Связанные продукты

pH 7.4 Dilution Buffer

Очистка

Purified by Nickel column.

Номер доступа

P21554

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Cannabinoid receptor 1 (CNR1/CB1) is a ~52 kDa protein. G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta(9)-tetrahydrocannabinol (THC). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain. Signaling typically involves reduction in cyclic AMP. In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses.

1. Ryberg, E. et al. (2005) FEBS letters 579, 259-64. PMID: 15620723

2. Hua, T. et al. (2016) Cell 167, 750-762.e14. PMID: 27768894

3. Shao, Z. et al. (2016) Nature 540, 602-606. PMID: 27851727

4. Yang, X. et al. (2022) Nature chemical biology 18, 831-840. PMID: 35637350

5. Gérard, CM. et al. (1991) The Biochemical journal 279 ( Pt 1), 129-34. PMID: 1718258

6. Oddi, S. et al. (2012) British journal of pharmacology 165, 2635-51. PMID: 21895628

7. Niehaus, JL. et al. (2007) Molecular pharmacology 72, 1557-66. PMID: 17895407

8. Jourdan, T. et al. (2013) Nature medicine 19, 1132-40. PMID: 23955712

Примечание

For research use only. Not suitable for clinical or therapeutic use.

Изображения

SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12)
Bioactivity

Detects recombinant CNR1 (Catalog No: HB822012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CNR1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CNR1 (Catalog No: HB822012)
Immunofluorescence

Immunofluorescence staining of SHSY5Y with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane，Cytoplasm.