









| カタログ番号 | HB941010 |
|---|---|
| 説明 |
InVivoMAb Anti-Human VEGFA (Iv0071) [Iv0071] (HB941010) is a human monoclonal antibody detecting Vascular endothelial growth factor A in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
|
| 種反応性 | Human |
| アプリケーション | ELISA, FCM, Neutralization |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1, kappa |
| クローンID | Iv0071 |
| テスト済みアプリケーション | FCM |
| ターゲット | L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor |
| エンドトキシンレベル | < 1 EU/mg |
| 純度 | >95% as determined by SDS-PAGE. |
| 関連製品 | pH 7.4 Dilution Buffer |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P15692 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |

SEC-HPLC detection for InVivoMAb Anti-Human VEGFA (ABV0265).

Flow-cytometry using anti-human VEGFA antibody.VEGFA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human VEGFA (Iv0071).

Flow-cytometry using FITC anti-human VEGFA antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA monoclonal antibody (Catalog: HB941010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using APC anti-human VEGFA antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human VEGFA monoclonal antibody (Catalog: HB941010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.










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