Der f 1 Allergen Recombinant Protein (C-His) (JN021011)

Name: Der f 1 Allergen Recombinant Protein (C-His)
Brand: abinScience
SKU: JN021011
Price: 264 USD
Availability: InStock

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100ug
1mg

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Даташит
SDS

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Каталожный номер	JN021011
Описание	Recombinant American house dust mite Der f 1/DERF1 Protein, C-His (JN021011) expressed in Insect Cells, spanning Met1-Met321. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	Insect cells
Номер доступа	P16311
Длина белка	Met1-Met321
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Dermatophagoides farinae (American house dust mite)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	37.35 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Peptidase 1, 3.4.22.65, Allergen Der f I, Major mite fecal allergen Der f 1, Der f 1, DERF1, Allergens
Фон	Peptidase 1 (Der f 1/DERF1) is a ~36 kDa protein. Thiol protease that hydrolyzes proteins, with a preference for Phe or basic residues.
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant American house dust mite Der f 1/DERF1 Protein, C-His
Bioactivity

Detects Dermatophagoides farinae DERF1/DERP1 Antibody (4C1) in indirect ELISAs.
Western blot

Detected by Anti-Der f 1 monoclonal antibody (Catalog No: HY546056)