Caenorhabditis elegans HECD-1 Recombinant Protein (N-Strep) (JN857011)

Name: Caenorhabditis elegans HECD-1 Recombinant Protein (N-Strep)
Brand: abinScience
SKU: JN857011
Price: 264 USD
Availability: InStock

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SDS

Vista general

Número de catálogo	JN857011
Descripción	Recombinant Caenorhabditis elegans HECD-1 Protein, N-Strep (JN857011) expressed in Insect Cells, spanning Met1-Asn2648. Purity: >90% by SDS-PAGE. Highlights ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	Insect cells
Número de acceso	V6CLA2
Longitud de proteína	Met1-Asn2648
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Caenorhabditis elegans
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	295.58 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	E3 ubiquitin-protein ligase hecd-1, 2.3.2.26, hecd-1, C34D4.14
Fondo	E3 ubiquitin-protein ligase hecd-1 is a ~292 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the ubiquitination and proteasomal-mediated degradation of cytoplasmic and mitochondrial proteins. Positively regulates lin-12 activity in the anchor cell (AC)/vulval precursor (VU) cell fate decision. Negatively regulates glp-1 activity in germline proliferation. May play a role in the formation of fibrous organelles, a hemidesmosome-like structure attaching muscles to the epidermis. 1. Liu, G. et al. (2011) The EMBO journal 30, 2990-3003. PMID: 21673654 2. Segref, A. et al. (2014) Cell metabolism 19, 642-52. PMID: 24703696 3. Chen, Y. et al. (2014) G3 (Bethesda, Md.) 5, 353-9. PMID: 25552605 4. Zahreddine, H. et al. (2010) Current biology : CB 20, 322-7. PMID: 20153198 5. Ackermann, L. et al. (2016) Nature structural & molecular biology 23, 995-1002. PMID: 27669035
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Caenorhabditis elegans HECD-1 Protein, N-Strep

Referencias

Datasheet

Caenorhabditis elegans HECD-1 Recombinant Protein (N-Strep).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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