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CHIKV Spike glycoprotein E2 Recombinant Protein (C-His) (VK572021)

CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)
CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)
CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)
CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)
CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)
CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерVK572021
Описание
Recombinant CHIKV Spike glycoprotein E2 Protein, C-His (VK572021) expressed in Mammalian Cells, spanning Ser326-Gln666. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииMammalian cells
Номер доступаAIL00833.1
Длина белкаSer326-Gln666
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыChikungunya virus (CHIKV)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 41.26 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияstructural polyprotein, structural polyprotein
Фон

Chikungunya virus (CHIKV) is an arthropod-borne alphavirus that causes Chikungunya fever, a re-emerging disease characterized by high fever, rash, and arthralgia. The E2 envelope glycoprotein is a structural protein that forms heterodimers with the E1 glycoprotein and is displayed on the viral surface as part of trimeric spike complexes. E2 is primarily responsible for receptor binding and host cell attachment, playing a key role in viral entry. As a major target of neutralizing antibodies, the E2 protein is widely used in serological diagnostics, vaccine development, and antiviral research.

Примечание For research use only.
Изображения
  • CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)

    Bioactivity

    Detected by anti-CHIKV spike glycoprotein E2 antibody (Catalog No: VK572043) in indirect ELISA.

  • CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant CHIKV Spike glycoprotein E2 Protein, C-His.

  • CHIKV Spike glycoprotein E2 Recombinant Protein (C-His)

    Western blot

    Detected by Anti-CHIKV spike glycoprotein E2 monoclonal antibody (Catalog No: VK572043)

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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